热烈祝贺我公司客户在PNAS上发表文章

热烈祝贺曹晓风课题组使用我公司的高通量测序平台在PNAS上发表题为“Dicer-like 3 producestransposable element-associated 24-nt siRNAs that control agricultural traitsin rice”的文章,并在表观遗传调控水稻重要农艺性状方面取得新进展。该研究得到了国家重点基础研究计划973、转基因专项和国家自然科学基金委项目的资助。

PNAS的影响因子为9.737,在SCI综合科学类排名第三位,因而已成为全球科研人员不可缺少的科研资料。

该文章通过对水稻进行小RNA测序,揭示了依赖OsDCL3a酶的24-nt小分子RNA主要通过调控转座子旁临基因的表达进而对水稻重要农艺性状精细调控。研究人员发现,水稻OsDCL3a RNAi株系表现出株高降低、叶夹角变大以及穗长变短的多效发育表型。依赖OsDCL3a的24-nt小分子RNA主要来源于水稻基因组上的重复序列,特别是MITE类转座子位点。这些24-nt小分子RNA通过介导H3K9me2等异染色质修饰调控旁邻基因的表达,其中包括多个调节赤霉素和油菜素内酯平衡的关键基因。这些基因在水稻株高和叶夹角等重要农艺形状的形态建成中发挥重要作用。该工作揭示了24-nt小分子RNA调控水稻基因组中的MITE类转座子及其附近基因表达的表观遗传调控基础。MITE类转座子在动、植物中广泛存在,该机制很可能不仅在水稻、玉米等农作物中是保守的,还可能为肿瘤发生等研究提供重要线索。

Dicer-like 3 producestransposable element-associated 24-nt siRNAs that control agricultural traitsin rice

6 回复
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  6. Vinny
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